Fig 1: General schematic of the miniswine implant model. Irradiation (IR) of the minipig is scheduled 90 days ~ [S—12 w] before the day of surgery [S—0] to induce hyposalivation. Saliva and plasma samples are collected post-IR [S—6 w] and [S—1] to assess IR-induced hyposalivation and to establish miniswine-specific baselines. The encapsulation of hS/PCs in implantable 3D-STs is timed for 2 weeks prior to surgery [S—2 w] to ensure cell growth and stability prior to shipment. The 3D-STs are shipped to Pittsburgh for implantation into the irradiated minipigs. Immunosuppression (IMS) regimens started on the day of surgery [S—0] as well as continuous care and oversight provided by the Allegheny General Hospital veterinary team. Saliva and plasma samples are also collected 2 days post-surgery [S + 2 days] and weekly [S + n w] for downstream human AMY1 and GLuc analysis. Tissue is harvested at terminal timepoints and shipped to Houston on wet ice for 3D-ST dissection and for control-tissue resections for L/D and IHC analysis.
Supplier Page from Biomatik for Human Alpha-Amylase (Amy1) Elisa Kit